Carbonic Anhydrase III Protein, Human, Recombinant (His Tag)

• Background:

  Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. Carbonic anhydrases (CAs) form a family of enzymes that catalyze the rapid conversion of carbon dioxide and water to bicarbonate and protons, a reaction that occurs rather slowly in the absence of a catalyst. The active site of most carbonic anhydrases contains a zinc ion, they are therefore classified as metalloenzymes. Several forms of carbonic anhydrase occur in nature. The primary function of the enzyme in animals is to interconvert carbon dioxide and bicarbonate to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide out of tissues. Plants contain a different form called β-carbonic anhydrase, which, from an evolutionary standpoint, is a distinct enzyme, but participates in the same reaction and also uses a zinc ion in its active site. Carbonic anhydrase 3, also known as Carbonate dehydratase III, CA-III and CA3, is a cytoplasm protein which belongs to thealpha-carbonic anhydrase family. CA3 is activated by proton donors such as imidazole and the dipeptide histidylhistidine. It is inhibited by coumarins and sulfonamide derivatives such as acetazolamide. At 6 weeks gestation, transcripts accumulate at low levels in the somites and at high levels throughout the notochord. As gestation continues, CA3 becomes abundant in all developing muscle masses and continues at high to moderate levels in the notochord.

• Synonyms:

  CAIII Protein, Human; Car3 Protein, Human

• Gene Name:

  Carbonic anhydrase III

• Expression System:

  E. coli

• Tag:

  C-His

• Endotoxin:

  Please contact us for more information.

• Purity:

  > 95 % as determined by SDS-PAGE.

• Activity:

  Measured by its esterase activity.The specific activity is >5 pmoles/min/μg.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile 50mM Tris, 500mM NaCl, 10% glycerol, pH 8.0 Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Enzyme Proteins

• Molecular Weight:

  The recombinant human CA3 consisting of 266 amino acids and has a calculated molecular mass of 30.4 kDa as estimated in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  CA3

• Species:

  Human

• Protein ID:

  NP_005172.1

• Overview:

  A DNA sequence encoding the human CA3 (NP_005172.1) (Met 1-Lys 260) was expressed, with a polyhistide tag at the C-terminus.

• AA Sequence:

  Met1-Lys260

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_005172.1