CD73 Protein, Human, Recombinant (His Tag)

• Background:

  5'-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein that belongs to the 5'-nucleotidase family. CD73 is a glycosylphosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signaling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1, and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purinergic receptors to induce cellular responses. CD73 has also been reported to regulate the expression of pro-inflammatory molecules in mouse endothelium.

• Synonyms:

  CALJA Protein, Human; CD73 Protein, Human; E5NT Protein, Human; eN Protein, Human; eNT Protein, Human; NT Protein, Human; NT5 Protein, Human; NTE Protein, Human

• Gene Name:

  5'-nucleotidase, ecto (CD73)

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Field of Research:

  Phosphatases and Regulators

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 85 % as determined by SDS-PAGE.

• Activity:

  1. Using the Octet RED System, the affinity constant (Kd) of CD73 Protein, Human, Recombinant (His Tag) (Cat. PH50503M2) bound MEDI9447 was 0.3 nM. 2. Measured by its ability to hydrolyze the 5’phosphate group from the substrate adenosine 5’monophosphate (AMP) . The specific activity is > 15,000 pmoles/min/μg.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile 20mM Tris, 120 mM NaCl, 4 mM CaCl2, 20 % glycerol, pH 7.5. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  CD Molecule proteins

• Molecular Weight:

  The secreted recombinant human NT5E consists of 532 amino acids with the predicted molecular mass of 59.2 kDa.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  NT5E

• Species:

  Human

• Protein ID:

  NP_002517.1

• Overview:

  A DNA sequence encoding the human NT5E (NP_002517.1) (Met1-Lys547) was expressed fused with a polyhistidine tag at the C-terminus.

• AA Sequence:

  Met1-Lys547

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_002517.1