XIAP Protein, Human, Recombinant (AVI Tag)

• Background:

  E3 ubiquitin-protein ligase XIAP / BIRC4, also known as an inhibitor of apoptosis protein 3, X-linked inhibitor of apoptosis protein, and IAP-like protein, is a protein that belongs to a family of apoptotic suppressor proteins. Members of this family share a conserved motif termed, baculovirus IAP repeat, which is necessary for their anti-apoptotic function. XIAP / BIRC4 functions through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 and inhibits apoptosis induced by menadione, a potent inducer of free radicals, and interleukin 1-beta converting enzyme. XIAP / BIRC4 also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. Mutations in this encoding gene are the cause of X-linked lymphoproliferative syndrome. Alternate splicing results in multiple transcript variants. Thought to be the most potent apoptosis suppressor, XIAP / BIRC4, directly binds and inhibits caspases -3, -7 and -9. Survivin, which also binds to several caspases, is up-regulated in a many tumour cell types. Defects in XIAP / BIRC4 are the cause of lymphoproliferative syndrome X-linked type 2 (XLP2) . XLP is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV) . Symptoms include severe or fatal mononucleosis, acquired hypogammaglobulinemia, pancytopenia and malignant lymphoma.

• Synonyms:

  API3 Protein, Human; BIRC4 Protein, Human; hIAP-3 Protein, Human; hIAP3 Protein, Human; IAP-3 Protein, Human; ILP1 Protein, Human; MIHA Protein, Human; XLP2 Protein, Human

• Gene Name:

  X-linked inhibitor of apoptosis, E3 ubiquitin protein ligase

• Expression System:

  E. coli

• Tag:

  C-AVI

• Endotoxin:

  Please contact us for more information.

• Purity:

  > 75 % as determined by SDS-PAGE.

• Activity:

  Measured by its binding ability in a functional ELISA.Immobilized recombinant human SMAC-His (Cat:PH51764E2) at 10 μg/ml (100 μl/well) can bind recombinant human XIAP-AVI (Cat:PH50215E9) with a linear range of 0.125-1.0 μg/ml.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile 25mM Tris, 10mM DTT, 1% glycerol, 0.2M Glutamine Potassium, pH 8.0 Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Enzyme Proteins

• Molecular Weight:

  The recombinant human XIAP comprises 253 amino acids and has a predicted molecular mass of 29.1 kDa as estimated in SDS-PAGE under non-reduced conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  XIAP

• Species:

  Human

• Protein ID:

  NP_001158.2

• Overview:

  A DNA sequence encoding the human XIAP (NP_001158.2) (Leu 121-Thr 356) was fused with an AVI tag at the C-terminus, and additonal two amino acids (Gly & Pro) at the N-terminus.

• AA Sequence:

  Leu121-Thr356

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_001158.2