LATS1 Antibody (Phospho-Thr1079) : Biotin (OABF01832-Biotin)

• Gene Name:

  Large tumor suppressor kinase 1

• Gene Aliases:

  Wts, WARTS

• Gene ID:

  9113

• Swiss Prot:

  O95835

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  This LATS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 1060-1100 amino acids derived from human LATS1 around the phosphorylation site of Thr1079

• Target:

  Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function.

• Clonality:

  Polyclonal

• Conjugation:

  Biotin

• Type:

  Polyclonal Antibody

• Applications:

  WB, IHC-P, IF

• Purification:

  Purified by Protein A.

• Concentration:

  1 mg/mL

• Format:

  Liquid. Purified antibody supplied in 1x PBS buffer.

• Reconstitution:

  All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

• Shipping Conditions:

  Wet Ice

• Fragment:

  IgG

• Specificity:

  This sequence will cross react with LATS2 if phosphorylated at Thr1041

• NCBI Gene Symbol:

  LATS1