PRKDC Antibody: Biotin (OABF01525-Biotin)

• Gene Name:

  Protein kinase, DNA-activated, catalytic polypeptide

• Gene Aliases:

  HYRC, p350, DNAPK, DNPK1, HYRC1, IMD26, XRCC7, DNAPKc, DNA-PKC, DNA-PKcs

• Gene ID:

  5591

• Swiss Prot:

  P78527

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  KLH conjugated synthetic peptide derived from human KAT2B

• Target:

  Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V (D) J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V (D) J recombination by activation of the hairpin endonuclease artemis (DCLRE1C) . The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D. Contributes to the determination of the circadian period length by antagonizing phosphorylation of CRY1 'Ser-588' and increasing CRY1 protein stability, most likely through an indirect machanism. Interacts with CRY1 and CRY2; negatively regulates CRY1 phosphorylation.

• Clonality:

  Polyclonal

• Conjugation:

  Biotin

• Type:

  Polyclonal Antibody

• Applications:

  IHC-P, IF

• Purification:

  Purified by Protein A.

• Concentration:

  1 mg/mL

• Format:

  Liquid. Purified antibody supplied in 1x PBS buffer.

• Reconstitution:

  All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

• Shipping Conditions:

  Wet Ice

• Fragment:

  IgG

• NCBI Gene Symbol:

  PRKDC