MDM2 Antibody (Phospho-Ser186+Ser188) : HRP (OAAF00265-HRP)

• Gene Name:

  E3 ubiquitin-protein ligase Mdm2

• Gene Aliases:

  HDMX, LSKB, hdm2, ACTFS

• Gene ID:

  4193

• Swiss Prot:

  Q00987

• Accession Number:

  NP_002383.2

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  The antiserum was produced against synthesized peptide derived between 151-200 amino acids from the Internal region of human MDM2 around the phosphorylation site of Ser186 and Ser188.

• Clonality:

  Polyclonal

• Conjugation:

  HRP: Horseradish Peroxidase

• Type:

  Polyclonal Antibody

• Applications:

  IF, ELISA

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration:

  0.6-0.7 mg/mL

• Dilution:

  The optimal working dilution should be determined by the end user.

• Format:

  Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.

• Reconstitution:

  All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

• Shipping Conditions:

  Wet Ice

• Specificity:

  MDM2 (Phospho-Ser186+Ser188) antibody detects endogenous levels of MDM2 only when phosphorylated at serine 186 and serine 188.

• Applications Notes:

  IF: 1:100~1:500; ELISA: 1:20000

• NCBI Gene Symbol:

  MDM2

• Protein Name:

  E3 ubiquitin-protein ligase Mdm2