BRAF Antibody (Phospho-Thr598) : HRP (OAAF00143-HRP)

• Gene Name:

  Serine/threonine-protein kinase B-raf

• Gene Aliases:

  NS7, B-raf, BRAF1, RAFB1, B-RAF1

• Gene ID:

  673

• Swiss Prot:

  P15056

• Accession Number:

  NP_004324.2

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  The antiserum was produced against synthesized peptide derived between 571-620 amino acids from the C-Terminal region of human B-RAF around the phosphorylation site of Thr599.

• Clonality:

  Polyclonal

• Conjugation:

  HRP: Horseradish Peroxidase

• Type:

  Polyclonal Antibody

• Applications:

  IHC, ELISA

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration:

  0.6-0.7 mg/mL

• Dilution:

  The optimal working dilution should be determined by the end user.

• Format:

  Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.

• Reconstitution:

  All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

• Shipping Conditions:

  Wet Ice

• Specificity:

  B-Raf (Phospho-Thr598) antibody detects endogenous levels of B-Raf only when phosphorylated at threonine 598.

• Applications Notes:

  IHC: 1:50~1:100; ELISA: 1:40000

• NCBI Gene Symbol:

  BRAF

• Protein Name:

  Serine/threonine-protein kinase B-raf