TOP2A Antibody - C-terminal region: FITC (AVARP04007_T100-FITC)

• Gene Name:

  Topoisomerase (DNA) II alpha 170kDa

• Gene Aliases:

  TOP2, TP2A, TOPIIA, TOP2alpha

• Gene ID:

  7153

• Swiss Prot:

  P11388

• Accession Number:

  NP_001058

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat, Dog, Guinea Pig, Horse, Pig, Rabbit

• Immunogen:

  The immunogen is a synthetic peptide directed towards the C terminal region of human TOP2A

• Target:

  TOP2A is a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromsome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.

• Partner Proteins:

  UBC; SUMO2; SUMO3; EIF6; RPA3; RPA2; RPA1; SUMO1; LMNA; CTNNB1; ITGA4; FN1; CSNK2A1; VCAM1; SRPK1; RB1; ESR1; TOP2B; CSNK2A2; UPF3B; PRKCG; PRKCB; PRKCA; TERF2; BLM; NEDD8; COPS5; CUL5; FBXW7; ISG15; CUL2; VHL; TCEB1; GSK3B; SUMO4; SMARCAD1; CTCF; RING1

• Clonality:

  Polyclonal

• Conjugation:

  FITC: Fluorescein Isothiocyanate

• Type:

  Polyclonal Antibody

• Applications:

  WB

• Sample Type:

  TOP2A is supported by BioGPS gene expression data to be expressed in HepG2

• Concentration:

  0.5 mg/mL

• Homology:

  Dog: 100%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 87%; Pig: 100%; Rabbit: 100%; Rat: 93%

• Format:

  Liquid. Purified antibody supplied in 1x PBS buffer.

• Reconstitution:

  All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

• Molecular Weight:

  174kDa

• Shipping Conditions:

  Wet Ice

• Protein Length:

  1531

• NCBI Gene Symbol:

  TOP2A

• Protein Name:

  DNA topoisomerase 2-alpha

• Nucleotide Accession Number:

  NM_001067