SETDB1 Antibody - N-terminal region : HRP (ARP38998_P050-HRP)

• Gene Name:

  SET domain, bifurcated 1

• Gene Aliases:

  ESET, KG1T, KMT1E, TDRD21, H3-K9-HMTase4

• Gene ID:

  9869

• Swiss Prot:

  Q15047-2

• Host:

  Rabbit

• Reactivity:

  Human, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit

• Immunogen:

  The immunogen is a synthetic peptide directed towards the N-terminal region of Human SETDB1

• Target:

  SETDB1 is a histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. SETDB1 mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. It probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins.

• Partner Proteins:

  MDM2; HSP90AA1; UBC; SKIL; LRIF1; BARD1; APLP1; TCERG1; TRIM28; VIM; SUMO2; PML; CLSTN1; JARID2; tat; MZT2B; FAM118B; MRPL44; LUC7L2; MAP4K5; TSC22D1; TTR; TK1; SMN1; PCYT2; PSME1; GRB7; CDKN1A; CDK4; BID; ANXA7; ZNF274; HIST1H3A; ESCO1; AKT2; AKT1; PPARG

• Clonality:

  Polyclonal

• Conjugation:

  HRP: Horseradish Peroxidase

• Type:

  Polyclonal Antibody

• Applications:

  WB

• Purification:

  Affinity Purified

• Concentration:

  0.5 mg/ml

• Homology:

  Cow: 92%; Dog: 93%; Guinea Pig: 86%; Horse: 93%; Human: 100%; Rabbit: 85%; Rat: 86%

• Format:

  Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.

• Reconstitution:

  All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

• Molecular Weight:

  43kDa

• Shipping Conditions:

  Wet Ice

• Protein Length:

  397

• NCBI Gene Symbol:

  SETDB1

• NCBI GB Accession Number:

  SETDB1

• Host or Source:

  Rabbit

• Protein Name:

  Histone-lysine N-methyltransferase SETDB1

• Peptide Sequence:

  PGCIGLDAATATVESEEIAELQQAVVEELGISMEELRHFIDEELEKMDCV