MCM4 Antibody - C-terminal region: HRP (ARP36663_T100-HRP)

• Gene Name:

  Minichromosome maintenance complex component 4

• Gene Aliases:

  NKCD, CDC21, CDC54, IMD54, NKGCD, hCdc21, P1-CDC21

• Gene ID:

  4173

• Swiss Prot:

  P33991

• Accession Number:

  NP_877423

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish

• Immunogen:

  The immunogen is a synthetic peptide directed towards the C terminal region of human MCM4

• Target:

  The protein encoded by MCM4 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported.

• Partner Proteins:

  HUWE1; UBC; PCNA; SUMO2; FKBP5; MCMBP; MDM2; POLK; CLUH; NCKAP1; CAP1; CAP2; EIF3B; MCM2; KPNA3; PRMT1; FKBP4; MCM7; MCM6; FBXW4; FBXO46; ITGA4; CDC5L; gag-pol; ORC6; CCNA1; VCAM1; DBF4; CDC7; LIG4; MCM5; MCM3; DCTN2; SF3A1; UBD; NEDD8; TONSL; SIRT7; ASF1

• Clonality:

  Polyclonal

• Conjugation:

  HRP: Horseradish Peroxidase

• Type:

  Polyclonal Antibody

• Applications:

  WB

• Sample Type:

  MCM4 is strongly supported by BioGPS gene expression data to be expressed in Jurkat

• Concentration:

  0.5 mg/mL

• Homology:

  Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 93%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 86%

• Format:

  Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.

• Reconstitution:

  All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

• Molecular Weight:

  95kDa

• Shipping Conditions:

  Wet Ice

• Protein Length:

  863

• NCBI Gene Symbol:

  MCM4

• Protein Name:

  DNA replication licensing factor MCM4

• Nucleotide Accession Number:

  NM_182746