Anti-Hu CD51/CD61 Biotin

• Description:

  CD51/CD61 (integrin alpha5beta3), also known as osteoclast functional antigen, serves as a vitronectin receptor, and binds also to fibronectin, fibrinogen, thrombospondin, osteopontin, collagen, and von Willebrand factor. Expression of this antigen increases with melanoma progression. In healthy individuals CD51/CD61 is expressed mainly on osteoclasts, placenta, and endothelial cells, at lower levels on platelets and macrophages.

• Certification:

  RUO

• Negative Species:

  Rabbit, Pig, Cow

• Immunogen:

  A cell suspension containing osteoclasts from osteoclastomas

• Target Antigen:

  CD51/CD61

• Clone:

  23C6

• Applications:

  FC (QC tested), IP, IHC-F

• Concentration:

  1 mg/mL

• Format:

  Biotin

• Buffer:

  Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Viertlboeck BC, Göbel TW: Chicken thrombocytes express the CD51/CD61 integrin. Vet Immunol Immunopathol. 2007 Sep 15;119 (1-2) :137-41, URL: https://pubmed.ncbi.nlm.nih.gov/17659354/, *Yamamoto T, Kurihara N, Yamaoka K, Ozono K, Okada M, Yamamoto K, Matsumoto S, Michigami T, Ono J, Okada S: Bone marrow-derived osteoclast-like cells from a patient with craniometaphyseal dysplasia lack expression of osteoclast-reactive vacuolar proton pump. J Clin Invest. 1993 Jan;91 (1) :362-7., URL: https://pubmed.ncbi.nlm.nih.gov/7678608/, *Leukocyte Typing IV., Knapp WB. et al. (Eds.), Oxford University Press (1989) ., URL: , *Davies J, Warwick J, Totty N, Philp R, Helfrich M, Horton M: The osteoclast functional antigen, implicated in the regulation of bone resorption, is biochemically related to the vitronectin receptor. J Cell Biol. 1989 Oct;109 (4 Pt 1) :1817-26. (IHC-F, IP, WB), URL: https://pubmed.ncbi.nlm.nih.gov/2477382/, *Barua A, Bitterman P, Bahr JM, Bradaric MJ, Hales DB, Luborsky JL, Abramowicz JS: Detection of tumor-associated neoangiogenesis by Doppler ultrasonography during early-stage ovarian cancer in laying hens: a preclinical model of human spontaneous ovarian cancer. J Ultrasound Med. 2010 Feb;29 (2) :173-82. (IHC), URL: https://pubmed.ncbi.nlm.nih.gov/20103787/

• Storage Conditions:

  Store at 2-8°C. Do not freeze.

• Specificity:

  The mouse monoclonal antibody 23C6 recognizes an extracellular epitope on CD51/CD61 complex, that is expressed mainly on human osteoclasts, but also e.g. on placenta, or melanoma cell lines. The epitope is native and sensitive to fixation. In chicken this antibody can be used to selectively identify the thrombocytes.

• Applications Notes:

  Flow cytometry: Recommended dilution: 1-5 μg/ml

• Species:

  Human, Chicken

• Isotype:

  Mouse IgG1