Human CD6 (T-cell differentiation antigen CD6) ELISA Kit

• Description:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CD6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CD6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CD6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CD6 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative:

  T12; TP120; Soluble CD6

• Gene Aliases:

  T12; TP120; Soluble CD6

• UniProt:

  P30203

• Reactivity:

  Human

• Field of Research:

  Signal transduction, Infection immunity

• Assay Type:

  Sandwich

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CD6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CD6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CD6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CD6 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  3.5 H

• Standard:

  2000 pg/mL

• Sample Type:

  Serum, plasma, tissue homogenates and other biological fluids

• Detection Range:

  31.25 - 2000 pg/mL

• Sensitivity:

  11.5 pg/mL

• Species:

  Human

• CAS Number:

  7732-18-5, 9000-83-3