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Human MT (Melatonin) Microsample ELISA Kit

CAT: 0855-ELK9164MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK9164MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
N-Acetyl-5-Methoxytryptamine
Reactivity
Human
Field of Research
Endocrinology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MT in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
300 pg/mL
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
4.69-300 pg/mL
Sensitivity
2.4 pg/mL

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