Mouse 4-HNE (4-Hydroxynonenal) Microsample ELISA Kit

• Product Name Alternative:

  4-HNE, HNE, 4-hydroxy-2-nonenal, 6-Hydroxynonenal

• Reactivity:

  Mouse

• Field of Research:

  Signal transduction, Metabolic pathway, Infection immunity, Hormone metabolism, Dermatology

• Assay Type:

  Sandwich

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse 4-HNE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse 4-HNE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse 4-HNE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse 4-HNE in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  3.5 H

• Standard:

  150 ng/mL

• Sample Type:

  Serum, plasma and other biological fluids.

• Detection Range:

  2.35 - 150 ng/mL

• Sensitivity:

  1.1 ng/mL