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Mouse CALB (Calbindin) Microsample ELISA Kit

CAT: 0855-ELK7713MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK7713MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
CAB27; Calbindin 1,28kDa; Calbindin D28; Vitamin D-dependent calcium-binding protein, avian-type
UniProt
P12658
Reactivity
Mouse
Field of Research
Metabolic pathway
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CALB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CALB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CALB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CALB in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
0.16-10 ng/mL
Sensitivity
0.066 ng/mL

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