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Human ERFE (Erythroferrone) Microsample ELISA Kit

CAT: 0855-ELK7647MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK7647MS-01Size:48 Tests
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Product Name Alternative
FAM132B; C1QTNF15; CTRP15; Myonectin; Family With Sequence Similarity 132, Member B; Complement C1q tumor necrosis factor-related protein 15
UniProt
Q4G0M1
Reactivity
Human
Field of Research
Signal transduction
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ERFE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ERFE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ERFE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ERFE in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
0.16-10 ng/mL
Sensitivity
0.052 ng/mL

UniProtKB · Q4G0M1

Erythroferrone

ERFE_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q4G0M1
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ERFE
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
W8S2M9
Protein keywords

PTM

Disulfide bondGlycoproteinHydroxylation

Molecular function

Hormone

Technical term

Proteomics identificationReference proteome

Cellular component

Secreted

Domain

Signal

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