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Mouse ADORA2b (Adenosine A2b Receptor) Microsample ELISA Kit

CAT: 0855-ELK6990MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK6990MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
ADORA2B; Adenosine receptor A2b
UniProt
Q60614
Reactivity
Mouse
Field of Research
CD & Adhesion molecule
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ADORA2b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ADORA2b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ADORA2b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ADORA2b in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
5000 pg/mL
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Detection Range
78.13-5000 pg/mL
Sensitivity
28 pg/mL

UniProtKB · Q60614

Adenosine receptor A2b

AA2BR_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q60614
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Adora2b
Protein existence
2: Evidence at transcript level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
—
Secondary accessions
Q8BXI2
Protein keywords

Cellular component

Cell membraneMembrane

PTM

Disulfide bondGlycoproteinLipoproteinPalmitate

Molecular function

G protein-coupled receptorReceptorTransducer

Technical term

Reference proteome

Domain

TransmembraneTransmembrane helix

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