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Mouse DUSP1 (Dual Specificity Phosphatase 1) Microsample ELISA Kit

CAT: 0855-ELK6611MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK6611MS-01Size:48 Tests
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Product Name Alternative
CL100; HVH1; MKP1; PTPN10; Mitogen-activated protein kinase phosphatase 1; Protein-tyrosine phosphatase CL100; Dual specificity protein phosphatase hVH1
UniProt
P28563
Reactivity
Mouse
Field of Research
Enzyme & Kinase
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse DUSP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse DUSP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse DUSP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse DUSP1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
2000 pg/mL
Sample Type
Serum, plasma, tissue homogenates and other biological fluids.
Detection Range
31.25-2000 pg/mL
Sensitivity
12.7 pg/mL

UniProtKB · P28563

Dual specificity protein phosphatase 1

DUS1_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P28563
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Dusp1
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
3.1.3.16, 3.1.3.48
Processing
—
Secondary accessions
—
Protein keywords

Biological process

Cell cycle

Molecular function

HydrolaseProtein phosphatase

Cellular component

Nucleus

PTM

PhosphoproteinUbl conjugation

Technical term

Reference proteome

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