Rat IL33 (Interleukin 33) Microsample ELISA Kit

CAT: 0855-ELK6541MS-01Size: 48 TestsDry Ice: NoHazardous: No

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CAT#:0855-ELK6541MS-01Size:48 Tests

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Product Name Alternative

IL-33; DV27; C9ORF26; IL1F11; NFHEV; Interleukin-1 Family, Member 11; Nuclear factor from high endothelial venules

UniProt

Q66H70

Reactivity

Rat

Field of Research

Cytokine; Infection immunity

Assay Type

Sandwich

Assay Principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IL33. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IL33. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IL33, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IL33 in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

3.5h

Standard

500 pg/mL

Sample Type

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Detection Range

7.82-500 pg/mL

Sensitivity

3 pg/mL

UniProtKB · Q66H70

Interleukin-33

IL33_RAT · Rattus norvegicus

View on UniProt ↗

Primary accession: Q66H70
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: Il33
Protein existence: 2: Evidence at transcript level
Organism: Rattus norvegicus (Rat)
Taxonomy ID: 10116
Alternative names: IL-33
EC number: —
Processing: Precursor
Secondary accessions: —

Protein keywords

Cellular component

ChromosomeCytoplasmCytoplasmic vesicleNucleusSecreted

Molecular function

Cytokine

Technical term

Reference proteome

Biological process

Transcription

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