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Mouse MATN2 (Matrilin 2) Microsample ELISA Kit

CAT: 0855-ELK6429MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK6429MS-01Size:48 Tests
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Product Name Alternative
Crtm2; MATN 2; Matn2; Matrilin-2; Matrilin2; MGC102477
UniProt
O08746
Reactivity
Mouse
Field of Research
Hematology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MATN2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MATN2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MATN2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MATN2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.16-10 ng/mL
Sensitivity
0.061 ng/mL

UniProtKB · O08746

Matrilin-2

MATN2_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
O08746
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Matn2
Protein existence
2: Evidence at transcript level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
E9QPK9, Q8R542
Protein keywords

Coding sequence diversity

Alternative splicing

Domain

Coiled coilEGF-like domainRepeatSignal

PTM

Disulfide bondGlycoprotein

Technical term

Reference proteome

Cellular component

Secreted

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