Products for Research Use Only

Rat GDF15 (Growth Differentiation Factor 15) Microsample ELISA Kit

CAT: 0855-ELK5775MS-01Size: 48 TestsDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0855-ELK5775MS-01Size:48 Tests
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Product Name Alternative
PDF; MIC1; NAG-1; PLAB; PTGFB; Macrophage inhibitory cytokine 1; NSAID-activated gene 1 protein; Placental bone morphogenetic protein; Prostate differentiation factor
UniProt
Q9Z0J6
Reactivity
Rat
Field of Research
Cytokine; Apoptosis; Tumor immunity; Infection immunity
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat GDF15. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GDF15. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat GDF15, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GDF15 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
1000 pg/mL
Sample Type
Serum, plasma, tissue homogenates and other biological fluids.
Detection Range
15.63-1000 pg/mL
Sensitivity
6 pg/mL

UniProtKB · Q9Z0J6

Growth/differentiation factor 15

GDF15_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
Q9Z0J6
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Gdf15
Protein existence
1: Evidence at protein level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
GDF-15
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoprotein

Molecular function

CytokineHormone

Technical term

Reference proteome

Cellular component

Secreted

Domain

Signal

Popular Products