Human LAB7-1 (B-Lymphocyte Activation Antigen B7-1) Microsample ELISA Kit

• Product Name Alternative:

  CD80; CD28LG; CD28LG1; BB1; B7-1 Antigen; T-lymphocyte activation antigen CD80; CTLA-4 counter-receptor B7.1

• Reactivity:

  Human

• Field of Research:

  CD & Adhesion molecule, Tumor immunity, Infection immunity, Immune molecule, Autoimmunity

• Assay Type:

  Sandwich

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LAB7-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LAB7-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LAB7-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LAB7-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  3.5 H

• Standard:

  5000 pg/mL

• Sample Type:

  Serum, plasma, tissue homogenates and other biological fluids

• Detection Range:

  78.13 - 5000 pg/mL

• Sensitivity:

  32 pg/mL