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Human CHIT1 (Chitinase 1) Microsample ELISA Kit

CAT: 0855-ELK4932MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK4932MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
CHI3; Chitotriosidase
UniProt
Q13231
Reactivity
Human
Field of Research
Enzyme & Kinase
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CHIT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CHIT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CHIT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CHIT1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
20 ng/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates and other biological fluids
Detection Range
0.32-20 ng/mL
Sensitivity
0.11 ng/mL

UniProtKB · Q13231

Chitotriosidase-1

CHIT1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q13231
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CHIT1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
3.2.1.14
Processing
Precursor
Secondary accessions
B3KNW6, J3KN09, Q0VGG5, Q0VGG6, Q3ZAR1, Q6ISC2, Q9H3V8, Q9UDJ8
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Biological process

Carbohydrate metabolismChitin degradationPolysaccharide degradation

Ligand

Chitin-binding

PTM

Disulfide bondGlycoprotein

Molecular function

GlycosidaseHydrolase

Cellular component

LysosomeSecreted

Domain

Signal

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