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Human ZYX (Zyxin) Microsample ELISA Kit

CAT: 0855-ELK4891MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK4891MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
ESP2; HED2
UniProt
Q15942
Reactivity
Human
Field of Research
Signal transduction; Developmental science
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ZYX. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ZYX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ZYX, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ZYX in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
0.16-10 ng/mL
Sensitivity
0.053 ng/mL

UniProtKB · Q15942

Zyxin

ZYX_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q15942
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ZYX
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
A4D2G6, B4DQX7, Q6I9S4
Protein keywords

PTM

AcetylationMethylationPhosphoprotein

Coding sequence diversity

Alternative splicing

Biological process

Cell adhesionHost-virus interaction

Cellular component

Cell junctionCytoplasmCytoskeletonNucleus

Technical term

Direct protein sequencingProteomics identificationReference proteome

Domain

LIM domainRepeat

Ligand

Metal-bindingZinc

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