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Mouse ITGa3 (Integrin Alpha 3) Microsample ELISA Kit

CAT: 0855-ELK3662MS-03Size: 5x 96 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK3662MS-03Size:5x 96 Tests
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Product Name Alternative
CD49c; CD49-C; ITG-A3; GAP-B3; GAPB3; MSK18; VCA-2; VL3A; VLA3a; FRP-2; Galactoprotein B3; Alpha 3 Subunit Of VLA-3 Receptor; CD49 antigen-like family member C
UniProt
Q62470
Reactivity
Mouse
Field of Research
CD & Adhesion molecule
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ITGa3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ITGa3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ITGa3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ITGa3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
20 ng/mL
Sample Type
Tissue homogenates and other biological fluids.
Detection Range
0.32-20 ng/mL
Sensitivity
0.12 ng/mL

UniProtKB · Q62470

Integrin alpha-3

ITA3_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q62470
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Itga3
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q08441, Q08442, Q5SWA8, Q5SWB9, Q6P6I1
Protein keywords

Coding sequence diversity

Alternative splicing

Biological process

Cell adhesion

Cellular component

Cell junctionCell membraneCell projectionMembrane

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoproteinLipoproteinPalmitate

Molecular function

IntegrinReceptor

Technical term

Reference proteome

Domain

RepeatSignalTransmembraneTransmembrane helix