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Human NPNT (Nephronectin) Microsample ELISA Kit

CAT: 0855-ELK3198MS-02Size: 96 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK3198MS-02Size:96 Tests
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Product Name Alternative
EGFL6L; POEM; Preosteoblast EGF-like repeat protein with MAM domain; Protein EGFL6-like
UniProt
Q6UXI9
Reactivity
Human
Field of Research
Kidney biomarker
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NPNT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NPNT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NPNT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NPNT in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Tissue homogenates and other biological fluids.
Detection Range
0.16-10 ng/mL
Sensitivity
0.05 ng/mL

UniProtKB · Q6UXI9

Nephronectin

NPNT_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q6UXI9
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
NPNT
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
A6NFT9, A8K1W4, B4DIT4, B4DYK3, B4E2H7, B4E3H2, D6RCA1, E9PCK8, E9PCQ1, E9PE64, E9PF04
Protein keywords

Coding sequence diversity

Alternative splicing

Ligand

Calcium

Biological process

Cell adhesionDifferentiation

Molecular function

Developmental protein

PTM

Disulfide bond

Domain

EGF-like domainRepeatSignal

Cellular component

Extracellular matrixSecreted

Technical term

Proteomics identificationReference proteome

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