Mouse CXCR4 (Chemokine C-X-C-Motif Receptor 4) Microsample ELISA Kit

• Product Name Alternative:

  CD184; Fusin; NPYR; HSY3RR; LAP3; LCR1; LESTR; NPY3R; NPYRL; WHIM; Leukocyte-derived seven transmembrane domain receptor; Lipopolysaccharide-associated protein 3

• UniProt:

  P70658

• Reactivity:

  Mouse

• Field of Research:

  Signal transduction, CD & Adhesion molecule, Tumor immunity, Infection immunity

• Assay Type:

  Sandwich

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CXCR4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CXCR4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CXCR4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CXCR4 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  3.5 H

• Standard:

  5000 pg/mL

• Sample Type:

  Tissue homogenates, cell lysates and other biological fluids

• Detection Range:

  78.13 - 5000 pg/mL

• Sensitivity:

  28 pg/mL