Mouse SCCA2 (Squamous Cell Carcinoma Antigen 2) Microsample ELISA Kit

• Product Name Alternative:

  SERPINB4; PI11; SPB4; Leupin; Serpin Peptidase Inhibitor, Clade B, Member 4; Peptidase Inhibitor 11

• UniProt:

  Q9Z2G2

• Reactivity:

  Mouse

• Field of Research:

  Tumor immunity, Gastroenterology

• Assay Type:

  Sandwich

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SCCA2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SCCA2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SCCA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SCCA2 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  3.5 H

• Standard:

  10 ng/mL

• Sample Type:

  Tissue homogenates, cell lysates and other biological fluids.

• Detection Range:

  0.16 - 10 ng/mL

• Sensitivity:

  0.057 ng/mL