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Mouse IL31 (Interleukin 31) Microsample ELISA Kit

CAT: 0855-ELK2827MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK2827MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
IL 31 ; IL-31
UniProt
Q6EAL8
Reactivity
Mouse
Field of Research
Cytokine; Tumor immunity; Infection immunity; Dermatology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL31. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL31. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IL31, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IL31 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
400 pg/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
6.25-400 pg/mL
Sensitivity
2.38 pg/mL

UniProtKB · Q6EAL8

Interleukin-31

IL31_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q6EAL8
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Il31
Protein existence
2: Evidence at transcript level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
IL-31
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Molecular function

Cytokine

PTM

Glycoprotein

Biological process

Immunity

Technical term

Reference proteome

Cellular component

Secreted

Domain

Signal

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