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Mouse TGFb3 (Transforming Growth Factor Beta 3) Microsample ELISA Kit

CAT: 0855-ELK2699MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK2699MS-01Size:48 Tests
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Product Name Alternative
TGF-B3; LAP; Latency-associated peptide
UniProt
P17125
Reactivity
Mouse
Field of Research
Cytokine; Tumor immunity; Infection immunity
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TGFb3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TGFb3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TGFb3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TGFb3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
500 pg/mL
Sample Type
Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
Detection Range
7.82-500 pg/mL
Sensitivity
2.9 pg/mL

UniProtKB · P17125

Transforming growth factor beta-3 proprotein

TGFB3_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P17125
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Tgfb3
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoproteinMethylation

Cellular component

Extracellular matrixSecreted

Molecular function

Growth factorMitogen

Technical term

Reference proteome

Domain

Signal

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