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Rat IgE (Immunoglobulin E) Microsample ELISA Kit

CAT: 0855-ELK2594MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK2594MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
IGHE; Immunoglobulin Heavy Constant Epsilon; Ig epsilon chain C region
Reactivity
Rat
Field of Research
Infection immunity; Immune molecule; Hematology; Autoimmunity
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IgE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IgE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IgE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IgE in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
2000 ng/mL
Sample Type
Serum, plasma, and other biological fluids
Detection Range
31.25-2000 ng/mL
Sensitivity
14.1 ng/mL

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