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Human IL1b (Interleukin 1 Beta) Microsample ELISA Kit

CAT: 0855-ELK1270MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK1270MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
IL-1B; IL1-B; IL1-Beta; IL1F2; IL-1β; Interleukin-1 Family Member 2; Catabolin
UniProt
P01584
Reactivity
Human
Field of Research
Cytokine; Tumor immunity; Infection immunity
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL1b in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
1000 pg/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
15.63-1000 pg/mL
Sensitivity
5.5 pg/mL

UniProtKB · P01584

Interleukin-1 beta

IL1B_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P01584
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
IL1B
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
IL-1 beta
EC number
—
Processing
Precursor
Secondary accessions
Q53X59, Q53XX2, Q7M4S7, Q7RU01, Q96HE5, Q9UCT6
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Molecular function

CytokineMitogenPyrogen

Cellular component

CytoplasmLysosomeSecreted

Biological process

Inflammatory response

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