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Rat APOB (Apolipoprotein B) Microsample ELISA Kit

CAT: 0855-ELK11145MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK11145MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
Apo-B; FLDB; Apo B-100; Apo B-48
UniProt
Q7TMA5
Reactivity
Rat
Field of Research
Metabolic pathway; Endocrinology; Cardiovascular biology; Hepatology; Nutrition metabolism
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat APOB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat APOB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat APOB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat APOB in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
500 μg/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
7.82-500 μg/mL
Sensitivity
2 μg/mL

UniProtKB · Q7TMA5

Apolipoprotein B-100

APOB_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
Q7TMA5
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Apob
Protein existence
1: Evidence at protein level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
Apo B-100
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

PTM

AcetylationDisulfide bondGlycoproteinLipoproteinPalmitatePhosphoprotein

Disease

Atherosclerosis

Biological process

Cholesterol metabolismLipid metabolismLipid transportSteroid metabolismSterol metabolismTransport

Cellular component

ChylomicronCytoplasmLDLLipid dropletSecretedVLDL

Molecular function

Heparin-binding

Technical term

Reference proteome

Coding sequence diversity

RNA editing

Domain

Signal

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