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Human PGRN (Progranulin) Microsample ELISA Kit

CAT: 0855-ELK10952MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK10952MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
PGRN; Acrogranin; GEP; GP88; GRN; PCDGF; PEPI; Proepithelin
UniProt
P28799
Reactivity
Human
Field of Research
Cytokine
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PGRN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PGRN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PGRN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PGRN in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
100 ng/mL
Sample Type
Serum, plasma and other biological fluids.
Detection Range
1.56-100 ng/mL
Sensitivity
0.52 ng/mL

UniProtKB · P28799

Progranulin

GRN_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P28799
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
GRN
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
PGRN
EC number
—
Processing
Precursor
Secondary accessions
D3DX55, P23781, P23782, P23783, P23784, Q53HQ8, Q53Y88, Q540U8, Q9BWE7, Q9H8S1, Q9UCH0
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Molecular function

Cytokine

PTM

Disulfide bondGlycoprotein

Cellular component

LysosomeSecreted

Disease

NeurodegenerationNeuronal ceroid lipofuscinosis

Domain

RepeatSignal

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