Pig PF4 (Platelet Factor 4) ELISA Kit

• Description:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig PF4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig PF4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig PF4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig PF4 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative:

  CXCL4; SCYB4; Chemokine C-X-C-Motif Ligand 4; Oncostatin-A; Iroplact

• Gene Aliases:

  CXCL4; SCYB4; Chemokine C-X-C-Motif Ligand 4; Oncostatin-A; Iroplact

• UniProt:

  P30034

• Reactivity:

  Pig

• Field of Research:

  Cytokine, Hematology

• Assay Type:

  Sandwich

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig PF4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig PF4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig PF4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig PF4 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  3.5 H

• Standard:

  1000 ng/mL

• Sample Type:

  Plasma.

• Detection Range:

  15.63 - 1000 ng/mL

• Sensitivity:

  6.5 ng/mL

• Species:

  Pig

• CAS Number:

  7732-18-5