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Mouse PF4 (Platelet Factor 4) ELISA Kit

CAT: 0855-ELK10224-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK10224-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PF4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PF4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PF4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PF4 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
CXCL4; SCYB4; Chemokine C-X-C-Motif Ligand 4; Oncostatin-A; Iroplact
UniProt
Q9Z126
Reactivity
Mouse
Field of Research
Cytokine; Hematology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PF4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PF4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PF4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PF4 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
1000 ng/mL
Sample Type
Serum, platelet-poor plasma and cell culture supernates
Detection Range
15.63-1000 ng/mL
Sensitivity
6.1 ng/mL

UniProtKB · Q9Z126

Platelet factor 4

PLF4_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q9Z126
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Pf4
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
PF-4
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Biological process

Chemotaxis

Molecular function

CytokineHeparin-binding

PTM

Disulfide bondGlycoproteinPhosphoprotein

Technical term

Reference proteome

Cellular component

Secreted

Domain

Signal

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