RegisterLogin

Mouse CFB (Complement Factor B) ELISA Kit

CAT: 0855-ELK10687-01Size: 48 TestsDry Ice: NoHazardous: No
CAT#: ELK10687-01Size: 48 Tests
Selected
PRICE
AVAILABILITY
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CFB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CFB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CFB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CFB in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
CF-B; BF; BFD; CFAB; GBG; H2-Bf; PBF2; B-Factor; Properdin; Properdin factor B; C3/C5 convertase; Glycine-rich beta glycoprotein
UniProt
P04186
Reactivity
Mouse
Field of Research
Infection immunity; Immune molecule; Hematology; Autoimmunity
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CFB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CFB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CFB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CFB in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
400 μg/mL
Sample Type
Serum, plasma and other biological fluids
Detection Range
6.25-400 μg/mL
Sensitivity
2.58 μg/mL

Related Products

CatalogName
ELK0029-01Simian CFB (Complement Factor B) ELISA Kit
ELK11103-01Human CFB (Complement Factor B) ELISA Kit
ELK10688-01Rat CFB (Complement Factor B) ELISA Kit
ELK10687MS-01Mouse CFB (Complement Factor B) Microsample ELISA Kit
ELK10688MS-01Rat CFB (Complement Factor B) Microsample ELISA Kit
ELK11123-01Mouse C4B (Complement C4-B) ELISA Kit
ELK10499-01Mouse CFD (Complement Factor D) ELISA Kit
ELK10320-01Mouse CFH (Complement Factor H) ELISA Kit
ELK1872-01Human C4B (Complement C4-B) ELISA Kit
ELK11123MS-01Mouse C4B (Complement C4-B) Microsample ELISA Kit

Alternative Products

CatalogName
MBS9420111-01Recombinant mouse Complement factor B
15851-05011Mouse Complement Factor D Antibody
Z06071-500Complement factor I, His, Mouse
MBS961611-01Recombinant Mouse Complement factor B
Z06071-100Complement factor I, His, Mouse
MBS768086-01Complement factor B Mouse Monoclonal
477870CFD (Complement Factor D, Complement Factor D (Adipsin) )
AP81549-01Complement factor B
546178Complement Factor B
546184Complement Factor H

Popular Products