Rat LRG1 (Leucine Rich Alpha-2-Glycoprotein 1) ELISA Kit

• Description:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat LRG1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat LRG1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat LRG1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat LRG1 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative:

  A2GL ; HMFT1766 ; Leucine rich alpha 2 glycoprotein ; Leucine-rich alpha-2-glycoprotein 1 ; LRG ; LRG1

• Gene Aliases:

  A2GL ; HMFT1766 ; Leucine rich alpha 2 glycoprotein ; Leucine-rich alpha-2-glycoprotein 1 ; LRG ; LRG1

• Reactivity:

  Rat

• Field of Research:

  Signal transduction, Tumor immunity, Infection immunity

• Assay Type:

  Sandwich

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat LRG1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat LRG1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat LRG1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat LRG1 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  3.5 H

• Standard:

  100 µg/mL

• Sample Type:

  Serum, plasma and other biological fluids.

• Detection Range:

  1.57 - 100 µg/mL

• Sensitivity:

  0.48 µg/mL

• Species:

  Rat

• CAS Number:

  7732-18-5