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Human INS (Insulin) Microsample ELISA Kit

CAT: 0855-ELK10617MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK10617MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
Insulin
UniProt
P01308
Reactivity
Human
Field of Research
Metabolic pathway; Endocrinology; Hormone metabolism
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human INS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human INS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human INS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human INS in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Serum, plasma, cell culture supernates and other biological fluids
Detection Range
0.16-10 ng/mL
Sensitivity
0.053 ng/mL

UniProtKB · P01308

Insulin

INS_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P01308
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
INS
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q5EEX2
Protein keywords

Technical term

3D-structureDirect protein sequencingPharmaceuticalProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Biological process

Carbohydrate metabolismGlucose metabolism

PTM

Cleavage on pair of basic residuesDisulfide bond

Disease

Diabetes mellitusDisease variant

Molecular function

Hormone

Cellular component

Secreted

Domain

Signal

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