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Mouse BSP (Bone Sialoprotein) ELISA Kit

CAT: 0855-ELK10590-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK10590-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse BSP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse BSP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse BSP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse BSP in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
IBSP; Bsp; BspII; SpII; Integrin-Binding Bone Sialoprotein; Cell-binding sialoprotein; Bone sialoprotein 2
UniProt
Q61711
Reactivity
Mouse
Field of Research
Metabolic pathway; Developmental science; Bone metabolism
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse BSP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse BSP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse BSP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse BSP in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
50 ng/mL
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
0.78-50 ng/mL
Sensitivity
0.21 ng/mL

UniProtKB · Q61711

Integrin-binding sialoprotein

SIAL_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q61711
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Ibsp
Protein existence
2: Evidence at transcript level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q61363, Q80VR6
Protein keywords

Biological process

BiomineralizationCell adhesion

PTM

GlycoproteinPhosphoproteinSulfation

Technical term

Reference proteome

Cellular component

Secreted

Ligand

Sialic acid

Domain

Signal

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