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Human TPSb2 (Tryptase Beta 2) Microsample ELISA Kit

CAT: 0855-ELK10559MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK10559MS-01Size:48 Tests
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Product Name Alternative
TPS2; Tryptase Beta II; Tryptase Beta III
UniProt
P20231
Reactivity
Human
Field of Research
Infection immunity
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TPSb2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TPSb2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TPSb2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TPSb2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
0.16-10 ng/mL
Sensitivity
0.062 ng/mL

UniProtKB · P20231

Tryptase beta-2

TRYB2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P20231
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
TPSB2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
Tryptase-2
EC number
3.4.21.59
Processing
Precursor
Secondary accessions
D2E6S0, D2E6S2, O95827, Q15664, Q9UQI6, Q9UQI7
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

Disulfide bondGlycoproteinPhosphoproteinZymogen

Molecular function

HydrolaseProteaseSerine protease

Cellular component

Secreted

Domain

Signal

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