Human APOH (Apolipoprotein H) ELISA Kit

• Description:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human APOH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human APOH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human APOH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human APOH in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative:

  Apo-H; B2G1; BG; B2-GP1; B2GP1; Previously; Beta 2 Glycoprotein 1; APC inhibitor; Activated protein C-binding protein; Anticardiolipin cofactor; Beta (2) GPI

• Gene Aliases:

  Apo-H; B2G1; BG; B2-GP1; B2GP1; Previously; Beta 2 Glycoprotein 1; APC inhibitor; Activated protein C-binding protein; Anticardiolipin cofactor; Beta(2)GPI

• UniProt:

  P02749

• Reactivity:

  Human

• Field of Research:

  Metabolic pathway, Cardiovascular biology

• Assay Type:

  Sandwich

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human APOH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human APOH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human APOH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human APOH in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  3.5 H

• Standard:

  400 ng/mL

• Sample Type:

  Serum, plasma and other biological fluids

• Detection Range:

  6.25 - 400 ng/mL

• Sensitivity:

  2.71 ng/mL

• Species:

  Human

• CAS Number:

  7732-18-5