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Mouse VF (Visfatin) Microsample ELISA Kit

CAT: 0855-ELK10321MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK10321MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
Nampt; NAmPRTase; PBEF; PBEF1; Pre-B-Cell Colony Enhancing Factor; Nicotinamide Phosphoribosyltransferase
UniProt
Q99KQ4
Reactivity
Mouse
Field of Research
Metabolic pathway; Cardiovascular biology; Hepatology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse VF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse VF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse VF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse VF in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
100 ng/mL
Sample Type
Serum, plasma and other biological fluids
Detection Range
1.57-100 ng/mL
Sensitivity
0.53 ng/mL

UniProtKB · Q99KQ4

Nicotinamide phosphoribosyltransferase

NAMPT_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q99KQ4
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Nampt
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
NAmPRTase, Nampt
EC number
2.4.2.12
Processing
—
Secondary accessions
Q8C3B5, Q9JKM0
Protein keywords

Technical term

3D-structureReference proteome

PTM

AcetylationPhosphoprotein

Biological process

Biological rhythmsPyridine nucleotide biosynthesis

Molecular function

CytokineGlycosyltransferaseTransferase

Cellular component

CytoplasmNucleusSecreted

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