Mouse PIEZO-1 (Piezo-Type mMechanosensitive iIon Channel Component 1) ELISA Kit
CAT:
855-ELK0282-01
Size:
48 Tests
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Mouse PIEZO-1 (Piezo-Type mMechanosensitive iIon Channel Component 1) ELISA Kit
Description:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PIEZO-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PIEZO-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PIEZO-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PIEZO-1 in the samples is then determined by comparing the OD of the samples to the standard curve.Product Name Alternative:
PIEZO1; FAM38A; KIAA0233Gene Aliases:
PIEZO1; FAM38A; KIAA0233UniProt:
E2JF22Reactivity:
MouseField of Research:
Signal transductionAssay Type:
SandwichAssay Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PIEZO-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PIEZO-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PIEZO-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PIEZO-1 in the samples is then determined by comparing the OD of the samples to the standard curve.Assay Performance Time:
3.5 HStandard:
5000 pg/mLSample Type:
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluidsDetection Range:
78.13 - 5000 pg/mLSensitivity:
22.3 pg/mLSpecies:
MouseCAS Number:
7732-18-5