Mouse PIEZO-1 (Piezo-Type mMechanosensitive iIon Channel Component 1) ELISA Kit

CAT:
855-ELK0282-01
Size:
48 Tests
  • Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
  • Dry Ice Shipment: No
Mouse PIEZO-1 (Piezo-Type mMechanosensitive iIon Channel Component 1) ELISA Kit - image 1

Mouse PIEZO-1 (Piezo-Type mMechanosensitive iIon Channel Component 1) ELISA Kit

  • Description:

    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PIEZO-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PIEZO-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PIEZO-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PIEZO-1 in the samples is then determined by comparing the OD of the samples to the standard curve.
  • Product Name Alternative:

    PIEZO1; FAM38A; KIAA0233
  • Gene Aliases:

    PIEZO1; FAM38A; KIAA0233
  • UniProt:

    E2JF22
  • Reactivity:

    Mouse
  • Field of Research:

    Signal transduction
  • Assay Type:

    Sandwich
  • Assay Principle:

    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PIEZO-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PIEZO-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PIEZO-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PIEZO-1 in the samples is then determined by comparing the OD of the samples to the standard curve.
  • Assay Performance Time:

    3.5 H
  • Standard:

    5000 pg/mL
  • Sample Type:

    Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Detection Range:

    78.13 - 5000 pg/mL
  • Sensitivity:

    22.3 pg/mL
  • Species:

    Mouse
  • CAS Number:

    7732-18-5