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Human STOM (Stomatin) ELISA Kit

CAT: 0855-ELK0269-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK0269-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human STOM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human STOM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human STOM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human STOM in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
Erythrocyte band 7 integral membrane protein; Erythrocyte membrane protein band 7.2; Protein 7.2b
UniProt
P27105
Reactivity
Human
Field of Research
Metabolic pathway
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human STOM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human STOM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human STOM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human STOM in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
20 ng/mL
Sample Type
Serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
0.32-20 ng/mL
Sensitivity
0.11 ng/mL

UniProtKB · P27105

Stomatin

STOM_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P27105
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
STOM
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
B1AM77, Q14087, Q15609, Q5VX96, Q96FK4
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Cellular component

Cell membraneCytoplasmCytoplasmic vesicleCytoskeletonMembrane

PTM

LipoproteinPalmitatePhosphoprotein