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Mouse KRT14 (Keratin 14) Microsample ELISA Kit

CAT: 0855-ELK0247MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK0247MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
CK14; EBS3; EBS4; K14; Cytokeratin 14; Epidermolysis Bullosa Simplex Dowling-Meara Koebner; Keratin, type I cytoskeletal 14
UniProt
Q61781
Reactivity
Mouse
Field of Research
Tumor immunity; Infection immunity
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse KRT14. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse KRT14. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse KRT14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse KRT14 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
0.16-10 ng/mL
Sensitivity
0.06 ng/mL

UniProtKB · Q61781

Keratin, type I cytoskeletal 14

K1C14_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q61781
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Krt14
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
—
Secondary accessions
A2A4G4, Q91VQ4, Q99LE0
Protein keywords

Domain

Coiled coil

Cellular component

CytoplasmIntermediate filamentKeratinNucleus

Technical term

Direct protein sequencingReference proteome

PTM

Disulfide bondPhosphoproteinUbl conjugation

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