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Mouse GREM2 (Gremlin 2) ELISA Kit

CAT: 0855-ELK0144-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK0144-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GREM2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GREM2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GREM2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GREM2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
Cysteine knot superfamily 1, BMP antagonist 2; Protein related to DAN and cerberus; PRDC; Cktsf1b2
UniProt
O88273
Reactivity
Mouse
Field of Research
Signal transduction; Tumor immunity; Developmental science; Bone metabolism
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GREM2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GREM2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GREM2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GREM2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
2000 pg/mL
Sample Type
Serum, plasma and other biological fluids
Detection Range
31.25-2000 pg/mL
Sensitivity
12.2 pg/mL

UniProtKB · O88273

Gremlin-2

GREM2_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
O88273
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Grem2
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Technical term

3D-structureReference proteome

Molecular function

CytokineDevelopmental proteinHeparin-binding

PTM

Disulfide bondGlycoprotein

Cellular component

Secreted

Domain

Signal

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