Recombinant Human NT-3

• Background:

  Neurotrophin-3 (NT-3) is a member of the NGF family of neurotrophic factors and is structurally related to β-NGF, BDNF and NT-4. The NT3 cDNA encodes a 257 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature NT3.The amino acid sequences of mature human, murine and rat NT-3 are identical. NT-3 selectively promotes the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous systems.

• Description:

  Recombinant Human Neurotrophin-3 is produced by our E.coli expression system and the target gene encoding Tyr139-Thr257 is expressed.

• Synonyms:

  Neurotrophin-3; NT-3; HDNF; Nerve Growth Factor 2; NGF-2; Neurotrophic Factor; NTF3

• UniProt:

  P20783

• Accession Number:

  P20783

• Endotoxin:

  Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test.

• Purity:

  Greater than 95% as determined by reducing SDS-PAGE.

• Reconstitution:

  Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

• Molecular Weight:

  13.6 KDa

• Shipping Conditions:

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

• Storage Conditions:

  Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Notes:

  Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Observed Molecular Weight:

  14 KDa, reducing conditions

• Other Product Names:

  Neurotrophin-3; NT-3; HDNF; Nerve Growth Factor 2; NGF-2; Neurotrophic Factor; NTF3

• CAS Number:

  9000-83-3