Recombinant Human AOC3 (C-6His)

• Background:

  Vascular adhesion protein-1 (VAP-1) is a copper amine oxidase with a topaquinone cofactor.VAP-1 is a type II integral membrane protein, but a soluble form of the enzyme is present in human serum, and its level increases in diabetes and some inflammatory liver diseases. VAP-1 catalyzes the oxidative deamination of small primary amines such as methylamine, benzylamine, and aminoacetone in a reaction that produces an aldehyde, ammonia, and H2O2. VAP-1 vascular expression is regulated at sites of inflammation through its release from intracellular granules in which the protein is stored. The adhesive function of VAP-1 has been demonstrated in studies showing that the protein is important for the adherence of certain lymphocyte subtypes to inflamed endothelial tissues. VAP-1 mediated adhesion is involved in the process of leukocyte extravasation, an important feature of inflammatory responses. VAP-1 is considered to be a therapeutic target for diabetes, oxidative stress, and inflammatory diseases.

• Description:

  Recombinant Human Membrane Primary Amine Oxidase is produced by our Mammalian expression system and the target gene encoding Arg28-Asn763 is expressed with a 6His tag at the C-terminus.

• Synonyms:

  Membrane primary amine oxidase; Copper amine oxidase; HPAO; Semicarbazide-sensitive amine oxidase; SSAO; Vascular adhesion protein 1; VAP-1; AOC3; VAP1

• UniProt:

  Q16853

• Accession Number:

  Q16853

• Endotoxin:

  Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.

• Purity:

  Greater than 95% as determined by reducing SDS-PAGE.

• Molecular Weight:

  82.6 KDa

• Shipping Conditions:

  The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.

• Storage Conditions:

  Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.

• Notes:

  Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Observed Molecular Weight:

  90 - 110 KDa, reducing conditions

• Other Product Names:

  Membrane primary amine oxidase; Copper amine oxidase; HPAO; Semicarbazide-sensitive amine oxidase; SSAO; Vascular adhesion protein 1; VAP-1; AOC3; VAP1

• CAS Number:

  9000-83-3