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Recombinant Human EIF1AX (N-6His)

CAT: 0855-EPT142Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0855-EPT142Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Eukaryotic Translation Initiation Factor 1A, X-Chromosomal (EIF1AX) is an essential eukaryotic translation initiation factor that belongs to the eIF-1A family. EIF1AX is required for the binding of the 43S complex (a 40S subunit, eIF2/GTP/Met-tRNAi and eIF3) to the 5' end of capped RNA and has been shown to interact with IPO13. EIF1AX contains one S1-like domain and seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA (I) to 40 S ribosomal subunits.
Description
Recombinant Human Eukaryotic Translation Initiation Factor 1A, X-Chromosomal is produced by our E.coli expression system and the target gene encoding Met1-Ile144 is expressed with a 6His tag at the N-terminus.
UniProt
P47813
Accession Number
P47813
Endotoxin
Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test.
Purity
Greater than 95% as determined by reducing SDS-PAGE.
Molecular Weight
18.6 KDa
Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Storage Conditions
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Storage Temperature
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Notes
Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Observed Molecular Weight
22 KDa, reducing conditions
Other Product Names
Eukaryotic Translation Initiation Factor 1A X-Chromosomal; eIF-1A X Isoform; Eukaryotic Translation Initiation Factor 4C; eIF-4C; EIF1AX; EIF1A; EIF4C

UniProtKB · P47813

Eukaryotic translation initiation factor 1A, X-chromosomal

IF1AX_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P47813
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
EIF1AX
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
eIF-1A X isoform, eIF1A X isoform
EC number
—
Processing
—
Secondary accessions
B2R5U5, Q0VGC2, Q5JPS5, Q5JPS6
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Cellular component

Cytoplasm

Biological process

Host-virus interactionProtein biosynthesis

Molecular function

Initiation factorRNA-bindingtRNA-binding

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